IVF was developed to treat infertility caused by blocked or damaged fallopian tubes. It is now used to treat a variety of infertility problems for suitable IVF candidates. The cost of IVF can vary dramatically, but Ami Hospital is happy to offer patient treatment packages that can help reduce costs and stress so that more people can experience the benefits of IVF.

IVF requires the stimulation of multiple follicles followed by aspiration of the eggs. These are placed with sperm in a laboratory dish to fertilize outside the body (in vitro). The fertilized eggs form embryos which are placed into the woman’s uterus or womb several days later, where implantation and embryo development can occur just as in any other normal pregnancy.

In vitro fertilization (IVF) is a four-step procedure:

Ovarian Stimulation and Monitoring

Egg (oocyte) Retrieval


Embryo Transfer

Step 1: Ovarian Stimulation and Monitoring

Various medications are used to stimulate the ovaries to produce multiple mature follicles- controlled ovarian hyperstimulation or superovulation, rather than the single egg normally developed each month. Follicles are cysts in the ovaries in which eggs grow to maturity.

Follicle-stimulating hormone (FSH) is the hormone used to stimulate the development of multiple eggs containing follicles. FSH is given by daily injection. The number of days and the dose will vary depending on follicle development. The response to FSH is monitored by ultrasound and hormone levels. When the follicles are fully developed, usually after 8 to 10 days, human chorionic gonadotropin (hCG) is given to release the eggs and trigger the final maturation of the eggs


Ultrasound uses sound waves to make sonar pictures of the body. Through ultrasound, the number and size of follicles can be observed.

Blood Tests

The response to FSH is monitored with frequent blood tests. Developing follicles secrete increasing amounts of the hormone estradiol. Along with ultrasound, estradiol levels are used to determine the optimal timing for the administration of hCG.

Step 2: Egg Retrieval

Eggs are retrieved by ultrasound-directed vaginal aspiration of ovarian follicles performed under narcoleptic analgesia, which is a combination of pain relievers and sedation. A needle is guided into each follicle in order to aspirate its contents. The egg along with the surrounding fluid is removed from the follicle. The fluid is immediately examined under a microscope. The process is repeated until all the mature follicles have been aspirated.

Step 3: Fertilization

A semen sample is obtained from the male partner and processed in our Infertility treatment Clinic at Vadodara, using laboratory techniques to obtain the strongest, most active sperm.

Prepared sperm are placed with one or more eggs in a laboratory dish for fertilization. After 3 to 5 days, if the eggs have successfully fertilized and are growing normally, they are transferred to the uterus. Not all eggs will be fertilized and not all fertilized eggs will continue to grow. In some cases, the embryos may be transferred as early as 2 days or as late as 6 days after retrieval. Some patients may choose to utilize intracytoplastic sperm injection during this step, a procedure which improves the chances of conception by placing a single sperm directly into a mature egg.

Step 4: Embryo Transfer

Embryo transfer is performed under ultrasound guidance without anesthesia. The embryos are placed in a catheter for transfer into the uterus.

By Ami Hospital-Infertility Treatment Center.

A-11/12, Cactus Park Society,

B/s Canara Bank,Nr. Abhilasha Char Rasta,New Sama Road,Vadodara (Guj)-390024

Ph: 0265 – 699-9007,  +91 851 117 1680

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